Partial listing of diffusion coefficients of integral membrane proteins (note that all are close to 10-9 to 10-10 cm2/sec).
| Method | Protein | D *109 cm2/s | References | |
| FRAP | EGF complexes | 8.5 | Hillman & Schlessinger Biochem. 21, 1667 (1982) | A-431 cells |
| FRAP | Beta receptors | 1.4 + 0.1 | Henis et al 1982 | Cultured hepatocytes |
| FRAP | B2AR | 4.0 + 1.2 | Barak et al 1997 | Cultured cells |
| FRAP | Vasopressin V2 receptor | 30 + 4.5 | Jans et al JCB 114, 53 (1991) | LLC-PK1 Renal epithelial cells |
| FRAP | GnRH- receptor | 1.2 – 1.6 | Nelson et al Endocrin. 140, 950 (1999) | Gonadotropes(anterior Pituitary) |
| FCS | Beta2AR | 1.0 + 0.5 | Hegener et al Biochem 43, 6190 (2004 | A549 |
| FCS | A1 AR agonist | 4.3 + 0.6 | Briddon et al. PNAS 101, 4673 | CHO – A1 |
| FCS | Glucocortico id receptor | 30 | Maier et al. JCS 118, 3353 (2005) | Pituitary cell membrane |
| FCS | GABA(A) ligand | 2.8 + 0.9, 0.14 +0.05 | Meissner et al., Biochem. 42, 1667 (2003) | Hippocampal neurons |
| FCS | Bradykinin receptor type 2 | 3.5 ± 0.6 | Philip et al. JBC 282, 19203 (2007) | HEK293 |
Hindered mobility is generally thought to be due to association with cytoskeleton proteins possessing diffusion coefficients between 0.19 and 4.4 ×10-2 
m2/s (1, 2), localization in clathrin-coated pits (3, 4, 5), internalization via clathrin-dependent endocytosis (6), or an association with lipid rafts with a D of 1 m2/s (7, 8), as well as with a reduced mobility inside the lipid rafts (maximum D
5 × 10-2 m2/s) (7). Note that single point FCS measurements cannot detect immobile fractions detected in many FRAP experiments , cannot be detected by single point FCS, and therefore, we cannot exclude the presence of an immobile receptor moiety.
Velazquez JL, Thompson CL, Barnes EM Jr, Angelides KJ. Distribution and lateral mobility of GABA/benzodiazepine receptors on nerve cells. J Neurosci. 1989 Jun;9(6):2163-9.
PMID: 2542487 | EndNote Citation
Peran M, Hicks BW, Peterson NL, Hooper H, Salas R. Lateral mobility and anchoring of recombinant GABAA receptors depend on subunit composition. Cell Motil Cytoskeleton. 2001 Oct;50(2):89-100.
PMID: 11746674 | EndNote Citation
Essrich C, Lorez M, Benson JA, Fritschy JM, Lüscher B. Postsynaptic clustering of major GABAA receptor subtypes requires the gamma 2 subunit and gephyrin. Nat Neurosci. 1998 Nov;1(7):563-71.
PMID: 10196563
Baer K, Essrich C, Benson JA, Benke D, Bluethmann H, Fritschy JM, Lüscher B. Postsynaptic clustering of gamma-aminobutyric acid type A receptors by the gamma3 subunit in vivo. Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12860-5.
PMID: 10536013 | PMCID: PMC23133 | EndNote Citation
Craig AM, Blackstone CD, Huganir RL, Banker G. Selective clustering of glutamate and gamma-aminobutyric acid receptors opposite terminals releasing the corresponding neurotransmitters. Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12373-7.
PMID: 7809044 | EndNote Citation
Kittler JT, Delmas P, Jovanovic JN, Brown DA, Smart TG, Moss SJ. Constitutive endocytosis of GABAA receptors by an association with the adaptin AP2 complex modulates inhibitory synaptic currents in hippocampal neurons J Neurosci. 2000 Nov 1;20(21):7972-7.
PMID: 11050117 | EndNote Citation
Pralle A, Keller P, Florin EL, Simons K, Hörber JK. Sphingolipid-cholesterol rafts diffuse as small entities in the plasma membrane of mammalian cells. J Cell Biol. 2000 Mar 6;148(5):997-1008.
PMID: 10704449 | PMC2174552 | EndNote Citation
Schütz GJ, Kada G, Pastushenko VP, Schindler H. Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy. EMBO J. 2000 Mar 1;19(5):892-901.
PMID: 10698931 | PMCID: PMC305629 | EndNote Citation